More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PCBP2 antibody [N1C1]
Immunoprecipitation of PCBP2 protein from Jurkat whole cell extracts using 5 μg of PCBP2 antibody [N1C1] (GTX114616). Western blot analysis was performed using PCBP2 antibody [N1C1] (GTX114616). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of PCBP2 protein from Jurkat whole cell extracts using 5 μg of PCBP2 antibody [N1C1] (GTX114616). Western blot analysis was performed using PCBP2 antibody [N1C1] (GTX114616). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of PCBP2 protein from Jurkat whole cell extracts using 5 μg of PCBP2 antibody [N1C1] (GTX114616). Western blot analysis was performed using PCBP2 antibody [N1C1] (GTX114616). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

PCBP2 antibody [N1C1]

GTX114616
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPCBP2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    PCBP2 antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5094
  • Target name
    PCBP2
  • Target description
    poly(rC) binding protein 2
  • Target synonyms
    HNRNPE2, HNRPE2, hnRNP-E2, poly(rC)-binding protein 2, alpha-CP2, epididymis secretory sperm binding protein, heterogeneous nuclear ribonucleoprotein E2, heterogenous nuclear ribonucleoprotein E2, hnRNP E2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15366
  • Protein Name
    Poly(rC)-binding protein 2
  • Scientific Description
    The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Colognori D, Sunwoo H, Kriz AJ, et al. Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X. Mol Cell. 2019,74(1):101-117.e10. doi: 10.1016/j.molcel.2019.01.015
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of PCBP2/hnRNP E2 using anti-PCBP2/hnRNP E2 antibody (A02425-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCBP2/hnRNP E2 antigen affinity purified polyclonal antibody (Catalog # A02425-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCBP2/hnRNP E2 at approximately 39KD. The expected band size for PCBP2/hnRNP E2 is at 39KD.
Product group Antibodies
Boster Bio
Anti-PCBP2/hnRNP E2 Antibody Picoband(r)A02425-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPCBP2
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PCBP2 Antibody144-02531
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPCBP2
  • SizePrice
Product group Antibodies
Biomatik
PCBP2 Polyclonal AntibodyCAC15132
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetPCBP2
  • SizePrice
Product group Antibodies
Bioss
PCBP2 Recombinant Antbody Kit (KD-Validated)BSM-90020R
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetPCBP2
  • SizePrice
Product group Antibodies
Assay Genie
PCBP2 AntibodyCAB2531
ReactivityHuman
TargetPCBP2
  • SizePrice
Anti-PCBP2 Antibody
Product group Antibodies
Antibodies.com
Anti-PCBP2 AntibodyA30576
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
IHC-P analysis of rat brain tissue using GTX33389 PCBP2 antibody. Dilution : 1:100
Product group Antibodies
GeneTex
PCBP2 antibodyGTX33389
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetPCBP2
  • SizePrice
Immunohistochemical staining of human fallopian tube shows mdoerate cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-PCBP2 AntibodyHPA038356
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetPCBP2
  • SizePrice
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate All lanes: PCBP2 antibody at 5.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 35, 36, 34 kDa Observed band size: 43 kDa
Product group Antibodies
Cusabio
PCBP2 AntibodyCSB-PA622995LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetPCBP2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
HNRNP-E2 / PCBP2 AntibodyLS-C672371
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetPCBP2
  • SizePrice