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PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody

ORB2315693
Biorbyt
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Yeast
TargetPCNA
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Overview

  • Supplier
    Biorbyt
  • Product Name
    PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Positive Control: Tonsil or reactive lymph node. SKBr3, HEK293 or U937 cells.. Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. IHC, WB
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PC5
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID5111
  • Target name
    PCNA
  • Target description
    proliferating cell nuclear antigen
  • Target synonyms
    ATLD2, proliferating cell nuclear antigen, DNA polymerase delta auxiliary protein, cyclin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP12004
  • Protein Name
    Proliferating cell nuclear antigen
  • Scientific Description
    PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody
  • Reactivity
    Human, Mouse, Rat, Yeast
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCNA using anti-PCNA antibody (A00125). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCNA antigen affinity purified polyclonal antibody (Catalog # A00125) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCNA at approximately 36 kDa. The expected band size for PCNA is at 29 kDa.
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