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ProductsBack/PD-L2 / PDCD1LG2 / CD273 Antibody

PD-L2 / PDCD1LG2 / CD273 Antibody

LS-C813924
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPDCD1LG2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PD-L2 / PDCD1LG2 / CD273 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:20000), IHC (1:50 - 1:300), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID80380
  • Target name
    PDCD1LG2
  • Target description
    programmed cell death 1 ligand 2
  • Target synonyms
    B7 dendritic cell molecule; B7DC; B7-DC; bA574F11.2; Btdc; butyrophilin B7-DC; CD273; PD-1-ligand 2; PDCD1 ligand 2; PDCD1L2; PDL2; PD-L2; programmed cell death 1 ligand 2; programmed death ligand 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of B7 DC using anti-B7 DC antibody (PB10010). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B7 DC antigen affinity purified polyclonal antibody (Catalog # PB10010) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B7 DC at approximately 37 kDa. The expected band size for B7 DC is at 31 kDa.
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