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ProductsBack/PD1 antibody [7A11B1]
WB analysis of A-20 cell lysate in the (A) absence and (B) presence of blocking recombinant protein. using GTX17271 PD1 antibody [7A11B1]. Working concentration : 1 microg/ml
WB analysis of A-20 cell lysate in the (A) absence and (B) presence of blocking recombinant protein. using GTX17271 PD1 antibody [7A11B1]. Working concentration : 1 microg/ml
WB analysis of A-20 cell lysate in the (A) absence and (B) presence of blocking recombinant protein. using GTX17271 PD1 antibody [7A11B1]. Working concentration : 1 microg/ml

PD1 antibody [7A11B1]

GTX17271
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPdcd1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PD1 antibody [7A11B1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 microg/mL. IHC-P: 2.5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7A11B1
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID18566
  • Target name
    Pdcd1
  • Target description
    programmed cell death 1
  • Target synonyms
    Ly101, PD-1, Pdc1, programmed cell death protein 1, mPD-1, programmed cell death 1 protein, protein PD-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ02242
  • Protein Name
    Programmed cell death protein 1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of PD1/Pdcd1 using anti-PD1/Pdcd1 antibody (A00178-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse EL-4 whole cell lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse RAW264.7 whole cell lysates, Lane 5: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD1/Pdcd1 antigen affinity purified polyclonal antibody (Catalog # A00178-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD1/Pdcd1 at approximately 55 kDa. The expected band size for PD1/Pdcd1 is at 32 kDa.
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