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ProductsBack/PDE10A Mouse Monoclonal Antibody [Clone ID: OTI1B2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE10A (RC211181, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE10A. Positive lysates LY416496 (100ug) and LC416496 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE10A (RC211181, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE10A. Positive lysates LY416496 (100ug) and LC416496 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE10A (RC211181, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE10A. Positive lysates LY416496 (100ug) and LC416496 (20ug) can be purchased separately from OriGene.

PDE10A Mouse Monoclonal Antibody [Clone ID: OTI1B2]

CF500635
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDE10A
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    PDE10A Mouse Monoclonal Antibody [Clone ID: OTI1B2]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1B2
  • Gene ID10846
  • Target name
    PDE10A
  • Target description
    phosphodiesterase 10A
  • Target synonyms
    ADSD2, HSPDE10A, IOLOD, LINC00473, PDE10A19, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A, 3',5' cAMP/cGMP phosphodiesterase, dJ416F21.1 (phosphodiesterase 10A), phosphodiesterase 10A1 (PDE10A1), phosphodiesterase 10A7 (PDE10A7)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y233
  • Protein Name
    cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
  • Scientific Description
    Carrier-free (BSA/glycerol-free) PDE10A mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PDE10A using anti-PDE10A antibody (A02605-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE10A antigen affinity purified polyclonal antibody (Catalog # A02605-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDE10A at approximately 100KD. The expected band size for PDE10A is at 100KD.
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