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Western blot analysis of extracts of A431 cell lines using PDGFB antibody.
Western blot analysis of extracts of A431 cell lines using PDGFB antibody.
Western blot analysis of extracts of A431 cell lines using PDGFB antibody.

PDGFB Antibody

CSB-PA017709KA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDGFB
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDGFB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5155
  • Target name
    PDGFB
  • Target description
    platelet derived growth factor subunit B
  • Target synonyms
    IBGC5, PDGF-2, PDGF2, SIS, SSV, c-sis, platelet-derived growth factor subunit B, PDGF subunit B, PDGF, B chain, becaplermin, epididymis secretory sperm binding protein, platelet-derived growth factor 2, platelet-derived growth factor B chain, platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog), platelet-derived growth factor, beta polypeptide (oncogene SIS), proto-oncogene c-Sis
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01127
  • Protein Name
    Platelet-derived growth factor subunit B
  • Scientific Description
    PDGF-BB is a cystine-linked homodimer PDGF family member with key roles in development, cell proliferation, cell survival, and angiogenesis. PDGF-BB is expressed by vascular endothelium, megakaryocytes and Leydig cells. PDGF-BB targets pericytes, fibroblasts, monocytes and other cell types. PDGF induces fibroblast growth and migration and is a chemoattractant for monocytes and granulocytes. Precursor PDGF-BB is cleaved intracellularly to generate a form that contains a carboxy-terminal stretch that serves to retain PDGF-BB in the extracellular matrix. In a second cleavage event, the carboxy-terminal stretch is removed extracellularly to generate mature PDGF-BB. PDGF-BB binds to PDGFRbeta and induces receptor dimerization. Signaling is through the PI3K/Akt, JNK, and PLCgamma pathways. PDGF-BB may have a role in some cancer types.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDGF beta using anti-PDGF beta antibody (A00348). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGF beta antigen affinity purified polyclonal antibody (Catalog # A00348) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGF beta at approximately 27KD. The expected band size for PDGF beta is at 27KD.
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Lane 1: Mouse Kidney lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Mouse Lung lysates; Lane 5: Rat Lung lysates; Lane 6: Human HUVEC cell lysates; Lane 7: Human A431 cell lysates; Lane 8: Human MCF-7 cell lysates; Lane 9: Human U-2OS cell lysates probed with PDGF B Polyclonal Antibody, Unconjugated (bs-0185R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss
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