IHC-P analysis of human colon carcinoma tissue using GTX25484 PDI antibody [RL77]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
![IHC-P analysis of human liver tissue using GTX25484 PDI antibody [RL77]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25484/GTX25484_1299_IHC-P_w_23060722_729.webp "IHC-P analysis of human liver tissue using GTX25484 PDI antibody [RL77]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human tonsil tissue using GTX25484 PDI antibody [RL77]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25484/GTX25484_1300_IHC-P_w_23060722_439.webp "IHC-P analysis of human tonsil tissue using GTX25484 PDI antibody [RL77]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![FACS analysis of 3T3 cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX25484/GTX25484_177_FACS_w_23060722_950.webp "FACS analysis of 3T3 cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde")
![FACS analysis of Hela cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX25484/GTX25484_178_FACS_w_23060722_124.webp "FACS analysis of Hela cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde")
![WB analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates using GTX25484 PDI antibody [RL77]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25484/GTX25484_1785_WB_w_23060722_339.webp "WB analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates using GTX25484 PDI antibody [RL77]. Dilution : 1:1000")
![FACS analysis of K562 cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX25484/GTX25484_179_FACS_w_23060722_657.webp "FACS analysis of K562 cells using GTX25484 PDI antibody [RL77] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde")
![ICC/IF analysis of MDCK cells using GTX25484 PDI antibody [RL77]. Green : Primary antibody Blue : cell nucleus Permeabilization : 0.1% Triton X-100 Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25484/GTX25484_673_ICC-IF_w_23060722_865.webp "ICC/IF analysis of MDCK cells using GTX25484 PDI antibody [RL77]. Green : Primary antibody Blue : cell nucleus Permeabilization : 0.1% Triton X-100 Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature")
![ICC/IF analysis of A549 cells using GTX25484 PDI antibody [RL77]. Green : Primary antibody Blue : cell nucleus](https://www.genetex.com/upload/website/prouct_img/normal/GTX25484/GTX25484_674_ICC-IF_w_23060722_600.webp "ICC/IF analysis of A549 cells using GTX25484 PDI antibody [RL77]. Green : Primary antibody Blue : cell nucleus")
![ICC/IF analysis of HMVEC Cells using GTX25484 PDI antibody [RL77]. Green : Primary antibody Blue : cell nucleus](https://www.genetex.com/upload/website/prouct_img/normal/GTX25484/GTX25484_675_ICC-IF_w_23060722_670.webp "ICC/IF analysis of HMVEC Cells using GTX25484 PDI antibody [RL77]. Green : Primary antibody Blue : cell nucleus")
IHC-P analysis of human colon carcinoma tissue using GTX25484 PDI antibody [RL77]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
PDI antibody [RL77]
GTX25484
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityCanine, Hamster, Human, Mouse, Porcine, Primate, Rat, Xenopus
TargetP4hb
Overview
- SupplierGeneTex
- Product NamePDI antibody [RL77]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:200-1:2000. ICC/IF: 1:100-1:1000. IHC-P: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRL77
- ConjugateUnconjugated
- Gene ID25506
- Target nameP4hb
- Target descriptionprolyl 4-hydroxylase subunit beta
- Target synonymsPDI, PDIR, protein disulfide-isomerase, Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide), cellular thyroid hormone-binding protein, prolyl 4-hydroxylase, beta polypeptide
- HostMouse
- IsotypeIgG2b
- Protein IDP04785
- Protein NameProtein disulfide-isomerase
- Scientific Descriptionmay be identical to protein disulfide isomerase, iodothyronine 5 monodeiodinase (5MD), and membrane associated thyroid hormone binding protein MTHBP. [RGD, Jun 2010]
- ReactivityCanine, Hamster, Human, Mouse, Porcine, Primate, Rat, Xenopus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Shen TJ, Chen CL, Tsai TT, et al. Hyperglycemia exacerbates dengue virus infection by facilitating poly(A)-binding protein-mediated viral translation. JCI Insight. 2022,7(21). doi: 10.1172/jci.insight.142805Read this paper
- Bauer CS, Cohen RN, Sironi F, et al. An interaction between synapsin and C9orf72 regulates excitatory synapses and is impaired in ALS/FTD. Acta Neuropathol. 2022,144(3):437-464. doi: 10.1007/s00401-022-02470-zRead this paper
Datasheet
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