ICC/IF analysis of NIH-3T3 cells using GTX22792 PDI antibody [RL90]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature Dilution : 1:75 for at least 1 hour at room temperature
![WB analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates using GTX22792 PDI antibody [RL90]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22792/GTX22792_1597_WB_w_23060620_256.webp "WB analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates using GTX22792 PDI antibody [RL90]. Dilution : 1:1000")
![ICC/IF analysis of MDCK cells using GTX22792 PDI antibody [RL90]. Permeabilization : 0.1% Triton X-100 Dilution : 10 μg/ml in blocking buffer for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22792/GTX22792_456_ICC-IF_w_23060620_539.webp "ICC/IF analysis of MDCK cells using GTX22792 PDI antibody [RL90]. Permeabilization : 0.1% Triton X-100 Dilution : 10 μg/ml in blocking buffer for at least 1 hour at room temperature")
![ICC/IF analysis of HMVEC Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibody Blue : Nuclei](https://www.genetex.com/upload/website/prouct_img/normal/GTX22792/GTX22792_458_ICC-IF_w_23060620_805.webp "ICC/IF analysis of HMVEC Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibody Blue : Nuclei")
![ICC/IF analysis of U2OS cells using GTX22792 PDI antibody [RL90]. Red : Primary antibody Green : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature Dilution : 1:75 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22792/GTX22792_461_ICC-IF_w_23060620_514.webp "ICC/IF analysis of U2OS cells using GTX22792 PDI antibody [RL90]. Red : Primary antibody Green : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature Dilution : 1:75 for at least 1 hour at room temperature")
![ICC/IF analysis of NS-1 Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibody Blue : Nuclei](https://www.genetex.com/upload/website/prouct_img/normal/GTX22792/GTX22792_459_ICC-IF_w_23060620_585.webp "ICC/IF analysis of NS-1 Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibody Blue : Nuclei")
![ICC/IF analysis of p19 Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibody Blue : Nuclei](https://www.genetex.com/upload/website/prouct_img/normal/GTX22792/GTX22792_460_ICC-IF_w_23060620_456.webp "ICC/IF analysis of p19 Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibody Blue : Nuclei")
![IHC-P analysis of human lung adenocarcinoma tissue using GTX22792 PDI antibody [RL90]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22792/GTX22792_1120_IHC-P_w_23060620_472.webp "IHC-P analysis of human lung adenocarcinoma tissue using GTX22792 PDI antibody [RL90]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human colon tissue using GTX22792 PDI antibody [RL90]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22792/GTX22792_1121_IHC-P_w_23060620_624.webp "IHC-P analysis of human colon tissue using GTX22792 PDI antibody [RL90]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![FACS analysis of Hela cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22792/GTX22792_144_FACS_w_23060620_218.webp "FACS analysis of Hela cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde")
ICC/IF analysis of NIH-3T3 cells using GTX22792 PDI antibody [RL90]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature Dilution : 1:75 for at least 1 hour at room temperature
PDI antibody [RL90]
GTX22792
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, Other Application
Product group Antibodies
ReactivityCanine, Hamster, Human, Mouse, Porcine, Primate, Rat
TargetP4hb
Overview
- SupplierGeneTex
- Product NamePDI antibody [RL90]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:200-1:2000. ICC/IF: 1:100. IHC-P: 1:100. IHC-Fr: 1:100. FACS: 0.5-1 microg/test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRL90
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID25506
- Target nameP4hb
- Target descriptionprolyl 4-hydroxylase subunit beta
- Target synonymsPDI, PDIR, protein disulfide-isomerase, Protein disulfide isomerase (Prolyl 4-hydroxylase, beta polypeptide), cellular thyroid hormone-binding protein, prolyl 4-hydroxylase, beta polypeptide
- HostMouse
- IsotypeIgG2a
- Protein IDP04785
- Protein NameProtein disulfide-isomerase
- Scientific Descriptionmay be identical to protein disulfide isomerase, iodothyronine 5 monodeiodinase (5MD), and membrane associated thyroid hormone binding protein MTHBP. [RGD, Jun 2010]
- ReactivityCanine, Hamster, Human, Mouse, Porcine, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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