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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA113942(PDP1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA113942(PDP1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA113942(PDP1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)

PDP1 Antibody

CSB-PA113942
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPDP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PDP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54704
  • Target name
    PDP1
  • Target description
    pyruvate dehydrogenase phosphatase catalytic subunit 1
  • Target synonyms
    PDH, PDP, PDPC, PDPC 1, PPM2A, PPM2C, pyruvate dehyrogenase phosphatase catalytic subunit 1, [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial, protein phosphatase 2C, magnesium-dependent, catalytic subunit, protein phosphatase, Mg2+/Mn2+ dependent 2A, pyruvate dehydrogenase (Lipoamide) phosphatase-phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0J1
  • Protein Name
    [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
  • Scientific Description
    Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PDP1/PDP using anti-PDP1/PDP antibody (A05607-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDP1/PDP antigen affinity purified polyclonal antibody (Catalog # A05607-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDP1/PDP at approximately 53-61 kDa. The expected band size for PDP1/PDP is at 61 kDa.
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