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ProductsBack/PDP1 antibody [N1N3]

PDP1 antibody [N1N3]

GTX109533
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPDP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PDP1 antibody [N1N3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID54704
  • Target name
    PDP1
  • Target description
    pyruvate dehydrogenase phosphatase catalytic subunit 1
  • Target synonyms
    [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial; PDH; PDP; PDP 1; PDPC; PDPC 1; PPM2A; PPM2C; protein phosphatase 2C, magnesium-dependent, catalytic subunit; protein phosphatase, Mg2+/Mn2+ dependent 2A; pyruvate dehydrogenase (Lipoamide) phosphatase-phosphatase; pyruvate dehyrogenase phosphatase catalytic subunit 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0J1
  • Protein Name
    [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
  • Scientific Description
    This gene encodes a protein similar to members of the protein phosphatase 2C (PP2C) family. Studies of the rat counterpart of this protein suggested that this protein may reside within the mitochondrial matrix space and be responsible for dephosphorylation and reactivation of the pyruvate dehydrogenase complex (PDC). [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDP1/PDP using anti-PDP1/PDP antibody (A05607-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDP1/PDP antigen affinity purified polyclonal antibody (Catalog # A05607-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDP1/PDP at approximately 53-61 kDa. The expected band size for PDP1/PDP is at 61 kDa.
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