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Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA364933 (PDP1 Antibody) at dilution 1/30 (Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA364933 (PDP1 Antibody) at dilution 1/30 (Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA364933 (PDP1 Antibody) at dilution 1/30 (Original magnification: x200)

PDP1 Rabbit Polyclonal Antibody

TA364933
OriGene
ApplicationsImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetPDP1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PDP1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID54704
  • Target name
    PDP1
  • Target description
    pyruvate dehydrogenase phosphatase catalytic subunit 1
  • Target synonyms
    [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial; PDH; PDP; PDP 1; PDPC; PDPC 1; PPM2A; PPM2C; protein phosphatase 2C, magnesium-dependent, catalytic subunit; protein phosphatase, Mg2+/Mn2+ dependent 2A; pyruvate dehydrogenase (Lipoamide) phosphatase-phosphatase; pyruvate dehyrogenase phosphatase catalytic subunit 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0J1
  • Protein Name
    [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
  • Scientific Description
    PDP1 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA113942(PDP1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of PDP1/PDP using anti-PDP1/PDP antibody (A05607-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDP1/PDP antigen affinity purified polyclonal antibody (Catalog # A05607-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDP1/PDP at approximately 53-61 kDa. The expected band size for PDP1/PDP is at 61 kDa.
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