IHC-P analysis of human pancreas carcinoma tissue using GTX83919 PDX1 antibody [2A12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![IHC-P analysis of human pancreas tissue using GTX83919 PDX1 antibody [2A12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83919/GTX83919_2020_IHC-P_w_23061420_793.webp "IHC-P analysis of human pancreas tissue using GTX83919 PDX1 antibody [2A12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![WB analysis of various samples using GTX83919 PDX1 antibody [2A12]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83919/GTX83919_3474_WB_w_23061420_736.webp "WB analysis of various samples using GTX83919 PDX1 antibody [2A12]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with PDX1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83919 PDX1 antibody [2A12]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83919/GTX83919_4003_WB_w_23061420_906.webp "WB analysis of HEK293T cells transfected with PDX1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83919 PDX1 antibody [2A12]. Loading : 5 ug per lane")
![ICC/IF analysis of HeLa cells transiently transfected with PDX1 plasmid using GTX83919 PDX1 antibody [2A12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83919/GTX83919_878_ICCIF_w_23061420_725.webp "ICC/IF analysis of HeLa cells transiently transfected with PDX1 plasmid using GTX83919 PDX1 antibody [2A12].")
![ICC/IF analysis of HT29 cells using GTX83919 PDX1 antibody [2A12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83919/GTX83919_879_ICCIF_w_23061420_902.webp "ICC/IF analysis of HT29 cells using GTX83919 PDX1 antibody [2A12].")
IHC-P analysis of human pancreas carcinoma tissue using GTX83919 PDX1 antibody [2A12]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
PDX1 antibody [2A12]
GTX83919
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPDX1
Overview
- SupplierGeneTex
- Product NamePDX1 antibody [2A12] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:20000. ICC/IF: 1:50-1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2A12
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3651
- Target namePDX1
- Target descriptionpancreatic and duodenal homeobox 1
- Target synonymsglucose-sensitive factor; GSF; IDX-1; insulin promoter factor 1, homeodomain transcription factor; insulin upstream factor 1; IPF1; IPF-1; islet/duodenum homeobox-1; IUF1; IUF-1; MODY4; PAGEN1; pancreas/duodenum homeobox protein 1; pancreatic-duodenal homeobox factor 1; PDX-1; somatostatin transcription factor 1; somatostatin-transactivating factor 1; STF-1
- HostMouse
- IsotypeIgG1
- Protein IDP52945
- Protein NamePancreas/duodenum homeobox protein 1
- Scientific DescriptionActivates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5-[CT]TAAT[TG]-3. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203