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ProductsBack/PDX1 antibody [5D5]
WB analysis of HEK293T cells transfected with PDX1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83918 PDX1 antibody [5D5]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with PDX1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83918 PDX1 antibody [5D5]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with PDX1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83918 PDX1 antibody [5D5]. Loading : 5 ug per lane

PDX1 antibody [5D5]

GTX83918
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPDX1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PDX1 antibody [5D5] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5D5
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3651
  • Target name
    PDX1
  • Target description
    pancreatic and duodenal homeobox 1
  • Target synonyms
    glucose-sensitive factor; GSF; IDX-1; insulin promoter factor 1, homeodomain transcription factor; insulin upstream factor 1; IPF1; IPF-1; islet/duodenum homeobox-1; IUF1; IUF-1; MODY4; PAGEN1; pancreas/duodenum homeobox protein 1; pancreatic-duodenal homeobox factor 1; PDX-1; somatostatin transcription factor 1; somatostatin-transactivating factor 1; STF-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP52945
  • Protein Name
    Pancreas/duodenum homeobox protein 1
  • Scientific Description
    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5-[CT]TAAT[TG]-3. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PDX1 using anti-PDX1 antibody (A00491-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDX1 antigen affinity purified polyclonal antibody (Catalog # A00491-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDX1 at approximately 42KD. The expected band size for PDX1 is at 42KD.
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