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ProductsBack/PE Anti-Rat CD4(domain 1) Antibody

PE Anti-Rat CD4(domain 1) Antibody

RD21273F
Reddot Biotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetCd4
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    PE Anti-Rat CD4(domain 1) Antibody
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX-38
  • Conjugate
    RPE
  • Gene ID24932
  • Target name
    Cd4
  • Target description
    Cd4 molecule
  • Target synonyms
    CD4 antigen; p55; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; W3/25; W3/25 antigen
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    CD4, also known as T4, is a 55kD glycoprotein member of the immunoglobin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD4 using anti-CD4 antibody (A00344-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates, Lane 2: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD4 antigen affinity purified polyclonal antibody (Catalog # A00344-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD4 at approximately 52KD. The expected band size for CD4 is at 52KD.
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