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PEBP1 Polyclonal Antibody

CAC14544
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPEBP1
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Overview

  • Supplier
    Biomatik
  • Product Name
    PEBP1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5037
  • Target name
    PEBP1
  • Target description
    phosphatidylethanolamine binding protein 1
  • Target synonyms
    epididymis luminal protein 210; epididymis secretory protein Li 34; epididymis secretory protein Li 96; HCNP; HCNPpp; HEL-210; HEL-S-34; HEL-S-96; hippocampal cholinergic neurostimulating peptide; neuropolypeptide h3; PBP; PEBP; PEBP-1; phosphatidylethanolamine-binding protein 1; prostatic binding protein; raf kinase inhibitor protein; Raf kinase inhibitory protein; RKIP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30086
  • Protein Name
    Phosphatidylethanolamine-binding protein 1
  • Scientific Description
    The PEBP1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PBP using anti-PBP antibody (A01668). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: mouse testis lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBP antigen affinity purified polyclonal antibody (Catalog # A01668) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PBP at approximately 21 kDa. The expected band size for PBP is at 21 kDa.
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