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PECAM-1 (RM0032-1D12)

SC-101454
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPecam1
100 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    PECAM-1 (RM0032-1D12)
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID18613
  • Target name
    Pecam1
  • Target description
    platelet/endothelial cell adhesion molecule 1
  • Target synonyms
    Cd31, PECAM-1, Pecam, platelet endothelial cell adhesion molecule
  • Host
    Rat
  • Isotype
    IgG2a
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of CD31 using anti-CD31 antibody (A01513-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31 antigen affinity purified polyclonal antibody (Catalog # A01513-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31 at approximately 120-130 kDa. The expected band size for CD31 is at 83 kDa.
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