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Perforin Antibody (YA3793, Mouse)

HY-P84096
MedChem Express
TargetPRF1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Perforin Antibody (YA3793, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5551
  • Target name
    PRF1
  • Target description
    perforin 1
  • Target synonyms
    cytolysin; HPLH2; lymphocyte pore-forming protein; P1; perforin 1 (pore forming protein); perforin-1; PFP
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Perforin Antibody (YA3793) is a mouse-derived and non-conjugated IgG1 antibody, targeting to PRF1. It can be applicated for WB, IHC-P, FC, ELISA assays, in the background of human, mouse.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Perforin using anti-Perforin antibody (PB9155). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human Perforin protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Perforin antigen affinity purified polyclonal antibody (Catalog # PB9155) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Perforin at approximately 40 kDa. The expected band size for Perforin is at 40 kDa.
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