More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PERK (phospho Ser555) antibody
WB analysis of HepG2 lysate using GTX00673 PERK (phospho Ser555) antibody preincubated with phospho- and non phospho- peptide.
WB analysis of HepG2 lysate using GTX00673 PERK (phospho Ser555) antibody preincubated with phospho- and non phospho- peptide.
WB analysis of HepG2 lysate using GTX00673 PERK (phospho Ser555) antibody preincubated with phospho- and non phospho- peptide.

PERK (phospho Ser555) antibody

GTX00673
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetEIF2AK3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    PERK (phospho Ser555) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9451
  • Target name
    EIF2AK3
  • Target description
    eukaryotic translation initiation factor 2 alpha kinase 3
  • Target synonyms
    PEK, PERK, WRS, eukaryotic translation initiation factor 2-alpha kinase 3, PKR-like ER kinase, PRKR-like endoplasmic reticulum kinase, pancreatic EIF2-alpha kinase, pancreatic eIF-2alpha kinase, protein tyrosine kinase EIF2AK3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NZJ5
  • Protein Name
    Eukaryotic translation initiation factor 2-alpha kinase 3
  • Scientific Description
    The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Mevalonate Pathway Enzyme HMGCS1 Contributes to Gastric Cancer Progression. Wang IH et al., 2020 Apr 27, Cancers (Basel)
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Anti-PERK Antibody
Product group Antibodies
Antibodies.com
Anti-PERK AntibodyA306749
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
RayBiotech
Anti-EIF2AK3 Antibody102-20239
ApplicationsWestern Blot
TargetEIF2AK3
  • SizePrice
Product group Antibodies
Biosynth
PERK antibody70R-17036
  • SizePrice
Figure 1. Western blot analysis of PERK using anti-PERK antibody (A01992-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: Human A431 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PERK antigen affinity purified polyclonal antibody (Catalog # A01992-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PERK at approximately 140KD. The expected band size for PERK is at 125KD.
Product group Antibodies
Boster Bio
Anti-PERK/EIF2AK3 Antibody Picoband(r)A01992-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetEIF2AK3
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-PERK Polyclonal Antibody, Unconjugated (bs-2469R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Product group Antibodies
References
Bioss
PERK Polyclonal AntibodyBS-2469R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetEIF2AK3
  • SizePrice
Western Blot analysis of Hela cells using PERK Polyclonal Antibody
Product group Antibodies
Cusabio
EIF2AK3 AntibodyCSB-PA003739
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetEIF2AK3
  • SizePrice
Product group Antibodies
Biomatik
Phospho-PERK (T981) Monoclonal AntibodyCAC12891
ApplicationsImmunoFluorescence, ELISA
TargetEIF2AK3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
EIF2AK3 / PERK AntibodyLS-C406669
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetEIF2AK3
  • SizePrice
ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04533 PERK (phospho Thr982) antibody. P-peptide : phospho-peptide N-peptide : non-phospho-peptide Peptides concentration : 1 μg/ml
Product group Antibodies
GeneTex
PERK (phospho Thr982) antibodyGTX04533
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetEIF2AK3
  • SizePrice
IHC-P analysis of human hepatocarcinoma using GTX81222 PERK antibody, N-term.
Product group Antibodies
References
GeneTex
PERK antibody, N-termGTX81222
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetEIF2AK3
  • SizePrice