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ProductsBack/Peroxiredoxin 6 Antibody (YA3154, Rabbit)

Peroxiredoxin 6 Antibody (YA3154, Rabbit)

HY-P83409
MedChem Express
TargetPRDX6
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Peroxiredoxin 6 Antibody (YA3154, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID9588
  • Target name
    PRDX6
  • Target description
    peroxiredoxin 6
  • Target synonyms
    1-Cys, AOP2, HEL-S-128m, LPCAT-5, NSGPx, PRX, aiPLA2, p29, peroxiredoxin-6, 1-Cys PRX, 1-Cys peroxiredoxin, 24 kDa protein, LPC acyltransferase 5, acidic calcium-independent phospholipase A2, antioxidant protein 2, epididymis secretory sperm binding protein Li 128m, glutathione-dependent peroxiredoxin, liver 2D page spot 40, lyso-PC acyltransferase 5, lysophosphatidylcholine acyltransferase 5, non-selenium glutathione peroxidase, red blood cells page spot 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Peroxiredoxin 6 Antibody (YA3154) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3154), targeting Peroxiredoxin 6, with a predicted molecular weight of 25 kDa (observed band size: 25 kDa). Peroxiredoxin 6 Antibody (YA3154) can be used for WB, IP experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRDX6 using anti-PRDX6 antibody (PB9350). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX6 antigen affinity purified polyclonal antibody (Catalog # PB9350) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDX6 at approximately 25 kDa. The expected band size for PRDX6 is at 25 kDa.
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