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PGC1 beta Monoclonal Antibody

CAB4331
Assay Genie
ReactivityHuman
Product group Antibodies
TargetPPARGC1B
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PGC1 beta Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID133522
  • Target name
    PPARGC1B
  • Target description
    PPARG coactivator 1 beta
  • Target synonyms
    ERRL1, PERC, PGC-1(beta), PGC1B, peroxisome proliferator-activated receptor gamma coactivator 1-beta, PGC-1-related estrogen receptor alpha coactivator, PPAR-gamma coactivator 1-beta, PPARAGCIbeta, PPARGC-1-beta, PPARgamma coactivator 1 beta, peroxisome proliferator-activated receptor gamma, coactivator 1 beta
  • Host
    Rabbit
  • Protein IDQ86YN6
  • Protein Name
    Peroxisome proliferator-activated receptor gamma coactivator 1-beta
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPARGC1B using anti-PPARGC1B antibody (A02933-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U2OS whole cell lysates. Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPARGC1B antigen affinity purified polyclonal antibody (Catalog # A02933-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPARGC1B at approximately 113KD. The expected band size for PPARGC1B is at 113KD.
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