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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA012670(PPARGC1B Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA012670(PPARGC1B Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA012670(PPARGC1B Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

PPARGC1B Antibody

CSB-PA012670
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPPARGC1B
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPARGC1B Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID133522
  • Target name
    PPARGC1B
  • Target description
    PPARG coactivator 1 beta
  • Target synonyms
    ERRL1, PERC, PGC-1(beta), PGC1B, peroxisome proliferator-activated receptor gamma coactivator 1-beta, PGC-1-related estrogen receptor alpha coactivator, PPAR-gamma coactivator 1-beta, PPARAGCIbeta, PPARGC-1-beta, PPARgamma coactivator 1 beta, peroxisome proliferator-activated receptor gamma, coactivator 1 beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86YN6
  • Protein Name
    Peroxisome proliferator-activated receptor gamma coactivator 1-beta
  • Scientific Description
    The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity. Three transcript variants encoding different isoforms have been found for this gene.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PPARGC1B using anti-PPARGC1B antibody (A02933-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U2OS whole cell lysates. Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPARGC1B antigen affinity purified polyclonal antibody (Catalog # A02933-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPARGC1B at approximately 113KD. The expected band size for PPARGC1B is at 113KD.
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