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PGE receptor EP1 (PTGER1) Antibody

CAB2913
Assay Genie
TargetPTGER1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PGE receptor EP1 (PTGER1) Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5731
  • Target name
    PTGER1
  • Target description
    prostaglandin E receptor 1
  • Target synonyms
    EP1, prostaglandin E2 receptor EP1 subtype, PGE receptor, EP1 subtype, PGE2 receptor EP1 subtype, prostaglandin E receptor 1 (subtype EP1), 42kD, prostaglandin E receptor 1 (subtype EP1), 42kDa, prostaglandin E receptor 1, subtype EP1, prostanoid EP1 receptor
  • Host
    Rabbit
  • Protein IDP34995
  • Protein Name
    Prostaglandin E2 receptor EP1 subtype
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Blank control (Black line): Mouse blood (Black)._x000D_ Primary Antibody (green line): PTGER1 Polyclonal Antibody (bs-6316R)_x000D_ Dilution: 3μg /10^6 cells;_x000D_ Isotype Control Antibody (orange line): Rabbit IgG ._x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG_x000D_ Dilution: 1μg /test._x000D_ Protocol_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Product group Antibodies
References
Bioss
PTGER1 Polyclonal AntibodyBS-6316R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPTGER1
  • SizePrice
Anti-PTGER1 Antibody
Product group Antibodies
Antibodies.com
Anti-PTGER1 AntibodyA100335
ApplicationsWestern Blot, ELISA
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Product group Antibodies
RayBiotech
Anti-Rat PTGER1 Antibody144-12310
ApplicationsWestern Blot
TargetPTGER1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-EP1 receptor / PTGER1EB07144
ApplicationsELISA, ImmunoHistoChemistry
TargetPTGER1
  • SizePrice
Product group Antibodies
GeneTex
Prostaglandin E Receptor EP1 antibodyGTX78161
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPTGER1
  • SizePrice
Figure 1. Western blot analysis of PTGER1 using anti-PTGER1 antibody (PA1583). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER1 antigen affinity purified polyclonal antibody (Catalog # PA1583) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER1 at approximately 42 kDa. The expected band size for PTGER1 is at 42 kDa.
Product group Antibodies
Boster Bio
Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody Picoband(r)PA1583-CARRIER-FREE
ApplicationsWestern Blot
TargetPTGER1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PTGER1 / EP1 AntibodyLS-C405554
ApplicationsWestern Blot, ELISA
TargetPTGER1
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Western Blot analysis of HepG2 cells using EP1 Polyclonal Antibody.
Product group Antibodies
Cusabio
PTGER1 AntibodyCSB-PA005788
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetPTGER1
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