Blank control (Black line): Mouse blood (Black)._x000D_ Primary Antibody (green line): PTGER1 Polyclonal Antibody (bs-6316R)_x000D_ Dilution: 3μg /10^6 cells;_x000D_ Isotype Control Antibody (orange line): Rabbit IgG ._x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG_x000D_ Dilution: 1μg /test._x000D_ Protocol_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.