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Blank control (Black line): Mouse blood (Black)._x000D_ Primary Antibody (green line): PTGER1 Polyclonal Antibody (bs-6316R)_x000D_ Dilution: 3μg /10^6 cells;_x000D_ Isotype Control Antibody (orange line): Rabbit IgG ._x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG_x000D_ Dilution: 1μg /test._x000D_ Protocol_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control (Black line): Mouse blood (Black)._x000D_ Primary Antibody (green line): PTGER1 Polyclonal Antibody (bs-6316R)_x000D_ Dilution: 3μg /10^6 cells;_x000D_ Isotype Control Antibody (orange line): Rabbit IgG ._x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG_x000D_ Dilution: 1μg /test._x000D_ Protocol_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control (Black line): Mouse blood (Black)._x000D_ Primary Antibody (green line): PTGER1 Polyclonal Antibody (bs-6316R)_x000D_ Dilution: 3μg /10^6 cells;_x000D_ Isotype Control Antibody (orange line): Rabbit IgG ._x000D_ Secondary Antibody (white blue line): Goat anti-rabbit IgG_x000D_ Dilution: 1μg /test._x000D_ Protocol_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

PTGER1 Polyclonal Antibody

BS-6316R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPTGER1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PTGER1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5731
  • Target name
    PTGER1
  • Target description
    prostaglandin E receptor 1
  • Target synonyms
    EP1; PGE receptor, EP1 subtype; PGE2 receptor EP1 subtype; prostaglandin E receptor 1 (subtype EP1), 42kD; prostaglandin E receptor 1 (subtype EP1), 42kDa; prostaglandin E receptor 1, subtype EP1; prostaglandin E2 receptor EP1 subtype; prostanoid EP1 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP34995
  • Protein Name
    Prostaglandin E2 receptor EP1 subtype
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Microsomal Prostaglandin E Synthase-1 Facilitates an Intercellular Interaction between CD4+ T Cells through IL-1beta Autocrine Function in Experimental Autoimmune Encephalomyelitis. Takemiya T et al., 2017 Dec 19, Int J Mol Sci
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