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ELISA analysis of antigen using GTX60752 PHC1 antibody [1F3F3]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
ELISA analysis of antigen using GTX60752 PHC1 antibody [1F3F3]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
ELISA analysis of antigen using GTX60752 PHC1 antibody [1F3F3]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng

PHC1 antibody [1F3F3]

GTX60752
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetPHC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PHC1 antibody [1F3F3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1F3F3
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1911
  • Target name
    PHC1
  • Target description
    polyhomeotic homolog 1
  • Target synonyms
    early development regulator 1 (homolog of polyhomeotic 1); early development regulatory protein 1; EDR1; HPH1; MCPH11; polyhomeotic-like 1; polyhomeotic-like protein 1; RAE28
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP78364
  • Protein Name
    Polyhomeotic-like protein 1
  • Scientific Description
    This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Neuronal necrosis is regulated by a conserved chromatin-modifying cascade. Liu K et al., 2014 Sep 23, Proc Natl Acad Sci U S A
    Read more

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Figure 1. Western blot analysis of PHC1 using anti-PHC1 antibody (A06437-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHC1 antigen affinity purified polyclonal antibody (Catalog # A06437-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHC1 at approximately 140 kDa. The expected band size for PHC1 is at 106,130 kDa.
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