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ProductsBack/PHEX / PEX Antibody (aa524-673)

PHEX / PEX Antibody (aa524-673)

LS-C681043
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetPHEX
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PHEX / PEX Antibody (aa524-673)
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    ELISA, IF (1:50 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    3.75 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5251
  • Target name
    PHEX
  • Target description
    phosphate regulating endopeptidase homolog X-linked
  • Target synonyms
    HPDR; HPDR1; HYP; HYP1; LXHR; metalloendopeptidase homolog PEX; PEX; phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets); phosphate-regulating neutral endopeptidase PHEX; vitamin D-resistant hypophosphatemic rickets protein; XLH; X-linked hypophosphatemia protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PHEX using anti-PHEX antibody (A02078). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse ovary tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: monkey kidney tissue lysates, Lane 7: rat NRK whole cell lysates, Lane 8: monkey COS-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHEX antigen affinity purified polyclonal antibody (Catalog # A02078) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHEX at approximately 86KD. The expected band size for PHEX is at 86KD.
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