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ProductsBack/PHEX Polyclonal Antibody, AbBy Fluor-350 Conjugated

PHEX Polyclonal Antibody, AbBy Fluor-350 Conjugated

BS-12313R-BF350
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetPHEX
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Overview

  • Supplier
    Bioss
  • Product Name
    PHEX Polyclonal Antibody, AbBy Fluor-350 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    FCM(1:20-100), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Other Conjugate
  • Gene ID5251
  • Target name
    PHEX
  • Target description
    phosphate regulating endopeptidase X-linked
  • Target synonyms
    HPDR, HPDR1, HYP, HYP1, LXHR, PEX, XLH, phosphate-regulating neutral endopeptidase PHEX, PHEX peptidase, X-linked hypophosphatemia protein, metalloendopeptidase homolog PEX, phosphate regulating endopeptidase homolog X-linked, phosphate regulating gene with homologies to endopeptidases on the X chromosome (hypophosphatemia, vitamin D resistant rickets), vitamin D-resistant hypophosphatemic rickets protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78562
  • Protein Name
    Phosphate-regulating neutral endopeptidase PHEX
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of PHEX using anti-PHEX antibody (A02078). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse ovary tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: monkey kidney tissue lysates, Lane 7: rat NRK whole cell lysates, Lane 8: monkey COS-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHEX antigen affinity purified polyclonal antibody (Catalog # A02078) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHEX at approximately 86KD. The expected band size for PHEX is at 86KD.
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