
ICC/IF analysis of L929 cells using GTX64499 PHGDH antibody. Blue : DAPI Dilution : 1:100
PHGDH antibody
GTX64499
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPHGDH
Overview
- SupplierGeneTex
- Product NamePHGDH antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID26227
- Target namePHGDH
- Target descriptionphosphoglycerate dehydrogenase
- Target synonyms3-PGDH, 3PGDH, HEL-S-113, NLS, NLS1, PDG, PGAD, PGD, PGDH, PHGDHD, SERA, D-3-phosphoglycerate dehydrogenase, 2-oxoglutarate reductase, 3-phosphoglycerate dehydrogenase, epididymis secretory protein Li 113, malate dehydrogenase
- HostRabbit
- IsotypeIgG
- Protein IDO43175
- Protein NameD-3-phosphoglycerate dehydrogenase
- Scientific DescriptionThis gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203




![Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 μg of PHGDH antibody [N1N2], N-term (GTX101948). Western blot analysis was performed using PHGDH antibody [N1N2], N-term (GTX101948). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101948/GTX101948_39834_20150407_IP_w_23060100_291.webp)
![Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101949/GTX101949_40604_20170818_WB_KO_watermark_w_23060100_485.webp)




