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ProductsBack/Phospho-Bad-S112 Rabbit mAb

Phospho-Bad-S112 Rabbit mAb

AP1371
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBAD
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-Bad-S112 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID572
  • Target name
    BAD
  • Target description
    BCL2 associated agonist of cell death
  • Target synonyms
    BBC2, BCL2L8, bcl2-associated agonist of cell death, BCL-X/BCL-2 binding protein, BCL2-antagonist of cell death protein, BCL2-binding component 6, BCL2-binding protein, bcl-2-binding component 6, bcl-2-like protein 8, bcl-XL/Bcl-2-associated death promoter, bcl2 antagonist of cell death, bcl2-L-8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92934
  • Protein Name
    Bcl2-associated agonist of cell death
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BAD using anti-BAD antibody (A03520-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: mouse Raw264.7 whole cell lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAD antigen affinity purified polyclonal antibody (Catalog # A03520-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAD at approximately 22 kDa. The expected band size for BAD is at 18 kDa.
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