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ProductsBack/Phospho-Camk2-T286 Rabbit mAb

Phospho-Camk2-T286 Rabbit mAb

AP1386
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCAMK2A
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-Camk2-T286 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID815
  • Target name
    CAMK2A
  • Target description
    calcium/calmodulin dependent protein kinase II alpha
  • Target synonyms
    CAMKA, CaMKIINalpha, CaMKIIalpha, MRD53, MRT63, calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, caM kinase II subunit alpha, caMK-II subunit alpha, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase type II alpha chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13554
  • Protein Name
    Calcium/calmodulin-dependent protein kinase type II subunit beta
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CaMKII alpha using anti-CaMKII alpha antibody (A03241-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CaMKII alpha antigen affinity purified polyclonal antibody (Catalog # A03241-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CaMKII alpha at approximately 50, 60KD. The expected band size for CaMKII alpha is at 54KD.
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