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Phospho-CBL (Tyr774) Antibody (YA1014, Rabbit)

HY-P81294
MedChem Express
TargetCBL
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Phospho-CBL (Tyr774) Antibody (YA1014, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID867
  • Target name
    CBL
  • Target description
    Cbl proto-oncogene
  • Target synonyms
    C-CBL, CBL2, FRA11B, NSLL, RNF55, E3 ubiquitin-protein ligase CBL, Cas-Br-M (murine) ecotropic retroviral transforming sequence, Cbl proto-oncogene, E3 ubiquitin protein ligase, RING finger protein 55, RING-type E3 ubiquitin transferase CBL, casitas B-lineage lymphoma proto-oncogene, fragile site, folic acid type, rare, fra(11)(q23.3), oncogene CBL2, proto-oncogene c-Cbl, signal transduction protein CBL
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Phospho-CBL (Tyr774) Antibody (YA1014) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1014), targeting Phospho-CBL (Tyr774), with a predicted molecular weight of 100 kDa (observed band size: 120 kDa). Phospho-CBL (Tyr774) Antibody (YA1014) can be used for WB, IP experiment in human, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CBL using anti-CBL antibody (A00152-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human CCRF-CEM whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human Hela whole cell lysates, Lane 9: rat testis tissue lysates, Lane 10: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBL antigen affinity purified polyclonal antibody (Catalog # A00152-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBL at approximately 120 kDa. The expected band size for CBL is at 120 kDa.
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