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Phospho-Cytokeratin 8 (KRT8)-S74 Rabbit pAb

AP1299
ABclonal
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetKRT8
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-Cytokeratin 8 (KRT8)-S74 Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3856
  • Target name
    KRT8
  • Target description
    keratin 8
  • Target synonyms
    CARD2, CK-8, CK8, CYK8, K2C8, K8, KO, keratin, type II cytoskeletal 8, cytokeratin-8, keratin 8, type II, type-II keratin Kb8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05787
  • Protein Name
    Keratin, type II cytoskeletal 8
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody (A01421). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: MCF-7 whole cell lysates, Lane 5: A549 whole cell lysates, Lane 6: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 8 antigen affinity purified polyclonal antibody (Catalog # A01421) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 8 at approximately 54KD, 56KD. The expected band size for Cytokeratin 8 is at 54KD.
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