More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Phospho-MAP2K1 (Ser221) Antibody
Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1(Phospho-Ser221) Antibody.
Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1(Phospho-Ser221) Antibody.
Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1(Phospho-Ser221) Antibody.

Phospho-MAP2K1 (Ser221) Antibody

CSB-PA439786
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMAP2K1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-MAP2K1 (Ser221) Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5604
  • Target name
    MAP2K1
  • Target description
    mitogen-activated protein kinase kinase 1
  • Target synonyms
    CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1, dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02750
  • Protein Name
    Dual specificity mitogen-activated protein kinase kinase 1
  • Scientific Description
    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases. Zebisch A, et al. (2006) Cancer Res; 66(7): 3401-8. Luciano BS, et al. (2006)J Biol Chem; 279(50): 52117-23. Wang X, et al. (2003) Oncogene; 22(1): 109-16. Gopalbhai K, et al. (2003) J Biol Chem; 278(10): 8118-25. Ling MT, et al. (2002)Oncogene; 21(55): 8498-505.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Biosynth
MEK1 antibody20R-2121
  • SizePrice
Product group Antibodies
RayBiotech
Anti-MAP2K1 Antibody144-00252
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Figure 1. Western blot analysis of MEK1/MAP2K1 using anti-MEK1/MAP2K1 antibody (A00292). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat lung tissue lysates, Lane 4: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK1/MAP2K1 antigen affinity purified polyclonal antibody (Catalog # A00292) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK1/MAP2K1 at approximately 45 kDa. The expected band size for MEK1/MAP2K1 is at 45 kDa.
Product group Antibodies
Boster Bio
Anti-MEK1/MAP2K1 Antibody Picoband(r)A00292-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with MEK1 antibody (GTX102391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Product group Antibodies
References
GeneTex
MEK1 antibodyGTX102391
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Human, Mouse, Rat
TargetMAP2K1
  • SizePrice
Product group Antibodies
Biomatik
MAP2K1 Polyclonal AntibodyCAC14738
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetMAP2K1
  • SizePrice
Product group Antibodies
Assay Genie
MEK1 AntibodyCAB0252
ReactivityHuman
TargetMAP2K1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-MEK1/2 Polyclonal Antibody, Unconjugated (bs-1041R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product group Antibodies
References
Bioss
MEK1 + MEK2 Polyclonal AntibodyBS-1041R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMAP2K1
  • SizePrice
Anti-MEK1 Antibody
Product group Antibodies
Antibodies.com
Anti-MEK1 AntibodyA96159
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Product group Antibodies
Atlas Antibodies
Anti-MAP2K1 AntibodyHPA026430
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetMAP2K1
  • SizePrice
Product group Antibodies
MedChem Express
MEK1 Antibody (YA300, Rabbit)HY-P80217
TargetMAP2K1
  • SizePrice