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Phospho-p95/NBS1 (Ser343) Antibody (YA165, Rabbit)

HY-P80473
MedChem Express
TargetNBN
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Phospho-p95/NBS1 (Ser343) Antibody (YA165, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4683
  • Target name
    NBN
  • Target description
    nibrin
  • Target synonyms
    AT-V1, AT-V2, ATV, NBS, NBS1, P95, hNbs1, nibrin, Nijmegen breakage syndrome 1 (nibrin), cell cycle regulatory protein p95, p95 protein of the MRE11/RAD50 complex
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Phospho-p95/NBS1 (Ser343) Antibody (YA165) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-p95/NBS1 (Ser343).
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p95 NBS1 antigen affinity purified polyclonal antibody (Catalog # PB9615) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p95 NBS1 at approximately 95 kDa. The expected band size for p95 NBS1 is at 85 kDa.
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