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Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody.The lane on the right is treated with the antigen-specific peptide.

Phospho-NCF1 (Ser345) Antibody

CSB-PA048500
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNCF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-NCF1 (Ser345) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID653361
  • Target name
    NCF1
  • Target description
    neutrophil cytosolic factor 1
  • Target synonyms
    CGD1, NCF-1, NCF-47K, NCF1A, NOXO2, SH3PXD1A, p47-phox, p47phox, neutrophil cytosol factor 1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NADPH oxidase organizer 2, SH3 and PX domain-containing protein 1A, neutrophil NADPH oxidase factor 1, neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1), nox organizer 2, nox-organizing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14598
  • Protein Name
    Neutrophil cytosol factor 1
  • Scientific Description
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989). Lomax K.J., Science 245:409-412(1989). Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of NCF1 using anti-NCF1 antibody (A01586-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human blood lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCF1 antigen affinity purified polyclonal antibody (Catalog # A01586-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCF1 at approximately 47KD. The expected band size for NCF1 is at 44KD.
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