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ProductsBack/Phospho-NF-kappaB2-S866/S870 Rabbit mAb

Phospho-NF-kappaB2-S866/S870 Rabbit mAb

AP1391
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetNFKB2
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-NF-kappaB2-S866/S870 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID4791
  • Target name
    NFKB2
  • Target description
    nuclear factor kappa B subunit 2
  • Target synonyms
    CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p49/p100, p52, nuclear factor NF-kappa-B p100 subunit, DNA-binding factor KBF2, NFKB, p52/p100 subunit, lymphocyte translocation chromosome 10 protein, nuclear factor Kappa-B, subunit 2, nuclear factor NF-kappa-B p52 subunit, nuclear factor of Kappa light chain gene enhancer in B cells 2, nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100), oncogene Lyt-10, transcription factor NFKB2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00653
  • Protein Name
    Nuclear factor NF-kappa-B p100 subunit
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFkB/NFKB2 p100/p52 antigen affinity purified polyclonal antibody (Catalog # PB9150) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFkB/NFKB2 p100/p52 at approximately 52 kDa (active form), 120kDa (precursor). The expected band size for NFkB/NFKB2 p100/p52 is at 97 kDa.
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