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Western Blot analysis of COS7 cells using Phospho-IkappaB-alpha (S32/S36) Polyclonal Antibody
Western Blot analysis of COS7 cells using Phospho-IkappaB-alpha (S32/S36) Polyclonal Antibody
Western Blot analysis of COS7 cells using Phospho-IkappaB-alpha (S32/S36) Polyclonal Antibody

Phospho-NFKBIA (S32/S36) Antibody

CSB-PA000559
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetNFKBIA
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-NFKBIA (S32/S36) Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4792
  • Target name
    NFKBIA
  • Target description
    NFKB inhibitor alpha
  • Target synonyms
    EDAID2, IKBA, MAD-3, NFKBI, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, ikB-alpha, major histocompatibility complex enhancer-binding protein MAD3, nuclear factor of kappa light chain gene enhancer in B-cells, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25963
  • Protein Name
    NF-kappa-B inhibitor alpha
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IKB Alpha using anti-IKB Alpha antibody (PB9291). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKB Alpha antigen affinity purified polyclonal antibody (Catalog # PB9291) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKB Alpha at approximately 39KD. The expected band size for IKB Alpha is at 36KD.
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