More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Phospho-PKC (pan) (betaII Ser660) Rabbit mAb

Phospho-PKC (pan) (betaII Ser660) Rabbit mAb

AP1414
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRKCA
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-PKC (pan) (betaII Ser660) Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID5578
  • Target name
    PRKCA
  • Target description
    protein kinase C alpha
  • Target synonyms
    AAG6, PKC-alpha, PKCA, PKCI+/-, PKCalpha, PRKACA, protein kinase C alpha type, PKC-A, aging-associated gene 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05771
  • Protein Name
    Protein kinase C beta type
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
PKC alpha AntibodyCAB0267
ReactivityHuman
TargetPRKCA
  • SizePrice
Western Blot analysis of MCF-7 cells using PKC Polyclonal Antibody
Product group Antibodies
Cusabio
PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCH/PRKCQ/PRKCZ AntibodyCSB-PA003800
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRKCA
  • SizePrice
Figure 1. Western blot analysis of PKC Alpha/PRKCA using anti-PKC Alpha/PRKCA antibody (A00743-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Alpha/PRKCA antigen affinity purified polyclonal antibody (Catalog # A00743-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC Alpha/PRKCA at approximately 80 kDa. The expected band size for PKC Alpha/PRKCA is at 77 kDa.
Product group Antibodies
Boster Bio
Anti-PKC Alpha/PRKCA Antibody Picoband(r)A00743-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPRKCA
  • SizePrice
Anti-PKC-pan Antibody
Product group Antibodies
Antibodies.com
Anti-PKC-pan AntibodyA94967
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Abbexa
PKC alpha (Phospho-Tyr657) AntibodyABX012471
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
MedChem Express
Phospho-PKC alpha (T638) Antibody (YA163, Rabbit)HY-P80279
TargetPRKCA
  • SizePrice
Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-PRKCA antibody. Corresponding PRKCA RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-PRKCA AntibodyHPA006564
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRKCA
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PRKCA / PKC-Alpha AntibodyLS-C402927
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetPRKCA
  • SizePrice
Product group Antibodies
Biomatik
Prkca Polyclonal AntibodyCAC09189
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetPRKCA
  • SizePrice
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-PKC alpha/beta II (Thr638/641) Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Product group Antibodies
References
Bioss
Phospho-PKC alpha (Thr638) Polyclonal AntibodyBS-3333R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Rat
TargetPRKCA
  • SizePrice