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ProductsBack/Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit mAb

Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit mAb

AP1325
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRPS6
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Overview

  • Supplier
    ABclonal
  • Product Name
    Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID6194
  • Target name
    RPS6
  • Target description
    ribosomal protein S6
  • Target synonyms
    S6, eS6, small ribosomal subunit protein eS6, 40S ribosomal protein S6, phosphoprotein NP33
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62753
  • Protein Name
    Small ribosomal subunit protein eS6
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPS6 using anti-RPS6 antibody (A01567-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: MCF-7 whole Cell lysates, Lane 4: A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS6 antigen affinity purified polyclonal antibody (Catalog # A01567-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS6 at approximately 32KD. The expected band size for RPS6 is at 29KD.
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