Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody [C1C3] (GTX108953). Western blot analysis was performed using PI3-Kinase p150 antibody [C1C3] (GTX108953). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody [C1C3] (GTX108953) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108953/GTX108953_40016_20161006_WB_shRNA_watermark_w_23060120_381.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody [C1C3] (GTX108953) diluted at 1:500.")
Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 μg of PI3-Kinase p150 antibody [C1C3] (GTX108953). Western blot analysis was performed using PI3-Kinase p150 antibody [C1C3] (GTX108953). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
PI3 kinase p150 antibody [C1C3]
GTX108953
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetPIK3R4
Overview
- SupplierGeneTex
- Product NamePI3 kinase p150 antibody [C1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID30849
- Target namePIK3R4
- Target descriptionphosphoinositide-3-kinase regulatory subunit 4
- Target synonymsVPS15, p150, phosphoinositide 3-kinase regulatory subunit 4, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, phosphatidylinositol 3-kinase-associated p150, phosphoinositide 3-kinase adaptor protein, phosphoinositide-3-kinase, regulatory subunit 4, p150
- HostRabbit
- IsotypeIgG
- Protein IDQ99570
- Protein NamePhosphoinositide 3-kinase regulatory subunit 4
- Scientific DescriptionRegulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Alkhoury C, Henneman NF, Petrenko V, et al. Class 3 PI3K coactivates the circadian clock to promote rhythmic de novo purine synthesis. Nat Cell Biol. 2023,25(7):975-988. doi: 10.1038/s41556-023-01171-3Read this paper
- Iershov A, Nemazanyy I, Alkhoury C, et al. The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARα. Nat Commun. 2019,10(1):1566. doi: 10.1038/s41467-019-09598-9Read this paper
- Nemazanyy I, Montagnac G, Russell RC, et al. Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling. Nat Commun. 2015,6:8283. doi: 10.1038/ncomms9283Read this paper
Datasheet
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