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PI3 kinase p150 Monoclonal Antibody

BSM-63077R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPIK3R4
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PI3 kinase p150 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), Flow-Cyt(1ug/Test), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID30849
  • Target name
    PIK3R4
  • Target description
    phosphoinositide-3-kinase regulatory subunit 4
  • Target synonyms
    p150; phosphatidylinositol 3-kinase-associated p150; phosphoinositide 3-kinase adaptor protein; phosphoinositide 3-kinase regulatory subunit 4; phosphoinositide-3-kinase, regulatory subunit 4, p150; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; VPS15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99570
  • Protein Name
    Phosphoinositide 3-kinase regulatory subunit 4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VPS15/PIK3R4 using anti-VPS15/PIK3R4 antibody (A06618-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VPS15/PIK3R4 antigen affinity purified polyclonal antibody (Catalog # A06618-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VPS15/PIK3R4 at approximately 150 kDa. The expected band size for VPS15/PIK3R4 is at 153 kDa.
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