![PI3 kinase p85 alpha antibody [N2C1], Internal immunoprecipitates PI3 kinase p85 alpha protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 5 μg of preimmune Rabbit IgG B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 μg PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) 10 % SDS-PAGE The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent] PI3 kinase p85 alpha antibody [N2C1], Internal immunoprecipitates PI3 kinase p85 alpha protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 5 μg of preimmune Rabbit IgG B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 μg PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) 10 % SDS-PAGE The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX111068/GTX111068_40912_IP_w_23060500_860.webp)
PI3 kinase p85 alpha antibody [N2C1], Internal immunoprecipitates PI3 kinase p85 alpha protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 5 μg of preimmune Rabbit IgG B. Immunoprecipitation of PI3 kinase p85 alpha protein by 5 μg PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) 10 % SDS-PAGE The immunoprecipitated PI3 kinase p85 alpha protein was detected by PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
PI3 kinase p85 alpha antibody [N2C1], Internal
GTX111068
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPIK3R1
Overview
- SupplierGeneTex
- Product NamePI3 kinase p85 alpha antibody [N2C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.46 mg/ml
- ConjugateUnconjugated
- Gene ID5295
- Target namePIK3R1
- Target descriptionphosphoinositide-3-kinase regulatory subunit 1
- Target synonymsAGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha, phosphatidylinositol 3-kinase regulatory subunit alpha, PI3 kinase-associated p85, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, growth factor receptor bound 1, phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha), phosphatidylinositol 3-kinase-associated p-85 alpha, phosphoinositide-3-kinase regulatory subunit alpha, phosphoinositide-3-kinase, regulatory subunit 1 (alpha), ptdIns-3-kinase regulatory subunit alpha
- HostRabbit
- IsotypeIgG
- Protein IDP27986
- Protein NamePhosphatidylinositol 3-kinase regulatory subunit alpha
- Scientific DescriptionPhosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111068/GTX111068_40912_20160505_WB_R_w_23060500_409.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111068/GTX111068_44146_20201211_WB_w_23060500_805.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111068/GTX111068_40912_20180817_WB_shRNA_watermark_w_23060500_622.webp)
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111068/GTX111068_40912_20160331_WB_M_w_23060500_564.webp)
![PI3 kinase p85 alpha antibody [N2C1], Internal detects PIK3R1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min PI3 kinase p85 alpha antibody [N2C1], Internal detects PIK3R1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. PI3 kinase p85 alpha antibody [N2C1], Internal (GTX111068) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX111068/GTX111068_40912_20160120_IHC-P_M_w_23060500_696.webp)





![PI3 kinase p85 alpha antibody [HL1398] detects PI3 kinase p85 alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1398] (GTX636838) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636838/GTX636838_T-44613_20220527_IHC-P_M_22062121_778.webp)
![PI3 kinase p85 alpha antibody [HL1414] detects PI3 kinase p85 alpha protein at Golgi apparatus and cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PI3 kinase p85 alpha stained by PI3 kinase p85 alpha antibody [HL1414] (GTX636880) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636880/GTX636880_T-44620_20220624_ICC_IF_22062919_235.webp)