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Western Blot analysis of KB cells using PI 3-kinase p85alpha Polyclonal Antibody
Western Blot analysis of KB cells using PI 3-kinase p85alpha Polyclonal Antibody
Western Blot analysis of KB cells using PI 3-kinase p85alpha Polyclonal Antibody

PIK3R1 Antibody

CSB-PA003765
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPIK3R1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PIK3R1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5295
  • Target name
    PIK3R1
  • Target description
    phosphoinositide-3-kinase regulatory subunit 1
  • Target synonyms
    AGM7; GRB1; IMD36; p85; p85-ALPHA; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3K regulatory subunit alpha; PI3-kinase subunit p85-alpha; ptdIns-3-kinase regulatory subunit alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27986
  • Protein Name
    Phosphatidylinositol 3-kinase regulatory subunit alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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H9C2 cells probed with Rabbit Anti-PI3 kinase p85 alpha subunit Polyclonal Antibody (bs-0128R) at 1:100 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1:100 (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)
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References
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Anti-PIK3R1 AntibodyHPA001216
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Figure 1. Western blot analysis of PI 3 Kinase p85 alpha/PIK3R1 using anti-PI 3 Kinase p85 alpha/PIK3R1 antibody (A00318-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI 3 Kinase p85 alpha/PIK3R1 antigen affinity purified polyclonal antibody (Catalog # A00318-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI 3 Kinase p85 alpha/PIK3R1 at approximately 85 kDa. The expected band size for PI 3 Kinase p85 alpha/PIK3R1 is at 85 kDa.
Product group Antibodies
References
Boster Bio
Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband(r)A00318-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetPIK3R1
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LifeSpan BioSciences
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