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ProductsBack/PI3 kinase p85 (phospho Tyr467) / p55 (phospho Tyr199) antibody
WB analysis of extracts from 293 cells untreated or treated with sorbitol using GTX50858 PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody.
WB analysis of extracts from 293 cells untreated or treated with sorbitol using GTX50858 PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody.
WB analysis of extracts from 293 cells untreated or treated with sorbitol using GTX50858 PI3 kinase p85 (phospho Tyr467)/p55 (phospho Tyr199) antibody.

PI3 kinase p85 (phospho Tyr467) / p55 (phospho Tyr199) antibody

GTX50858
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetPIK3R1
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Overview

  • Supplier
    GeneTex
  • Product Name
    PI3 kinase p85 (phospho Tyr467) / p55 (phospho Tyr199) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5295
  • Target name
    PIK3R1
  • Target description
    phosphoinositide-3-kinase regulatory subunit 1
  • Target synonyms
    AGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha, phosphatidylinositol 3-kinase regulatory subunit alpha, PI3 kinase-associated p85, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, growth factor receptor bound 1, phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha), phosphatidylinositol 3-kinase-associated p-85 alpha, phosphoinositide-3-kinase regulatory subunit alpha, phosphoinositide-3-kinase, regulatory subunit 1 (alpha), ptdIns-3-kinase regulatory subunit alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00459
  • Protein Name
    Phosphatidylinositol 3-kinase regulatory subunit beta
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Li K, Liu X, Hou R, et al. Uncovering mechanisms of Baojin Chenfei formula treatment for silicosis by inhibiting inflammation and fibrosis based on serum pharmacochemistry and network analysis. Ecotoxicol Environ Saf. 2023,260:115082. doi: 10.1016/j.ecoenv.2023.115082
    Read this paper
  • Tanaka S, Hosogi S, Sawabe Y, et al. PPARα induced NOS1 phosphorylation via PI3K/Akt in guinea pig antral mucous cells: NO-enhancement in Ca(2+)-regulated exocytosis. Biomed Res. 2016,37(3):167-78. doi: 10.2220/biomedres.37.167
    Read this paper

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Figure 1. Western blot analysis of PI 3 Kinase p85 alpha/PIK3R1 using anti-PI 3 Kinase p85 alpha/PIK3R1 antibody (A00318-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI 3 Kinase p85 alpha/PIK3R1 antigen affinity purified polyclonal antibody (Catalog # A00318-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI 3 Kinase p85 alpha/PIK3R1 at approximately 85 kDa. The expected band size for PI 3 Kinase p85 alpha/PIK3R1 is at 85 kDa.
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