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Western Blot analysis of HuvEc cells using PIAS 1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using PIAS 1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using PIAS 1 Polyclonal Antibody

PIAS1 Antibody

CSB-PA003776
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPIAS1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PIAS1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8554
  • Target name
    PIAS1
  • Target description
    protein inhibitor of activated STAT 1
  • Target synonyms
    AR interacting protein; DDXBP1; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box-binding protein 1; E3 SUMO-protein ligase PIAS1; E3 SUMO-protein transferase PIAS1; GBP; GU/RH-II; gu-binding protein; protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein; zinc finger, MIZ-type containing 3; ZMIZ3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75925
  • Protein Name
    E3 SUMO-protein ligase PIAS1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PIAS1 using anti-PIAS1 antibody (A01707). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS1 antigen affinity purified polyclonal antibody (Catalog # A01707) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS1 at approximately 72KD. The expected band size for PIAS1 is at 72KD.
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