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PIAS1 Antibody (YA2217, Rabbit)

HY-P82472
MedChem Express
TargetPIAS1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    PIAS1 Antibody (YA2217, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8554
  • Target name
    PIAS1
  • Target description
    protein inhibitor of activated STAT 1
  • Target synonyms
    DDXBP1, GBP, GU/RH-II, ZMIZ3, E3 SUMO-protein ligase PIAS1, AR interacting protein, DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1, DEAD/H box-binding protein 1, E3 SUMO-protein transferase PIAS1, RNA helicase II-binding protein, gu-binding protein, protein inhibitor of activated STAT protein 1, zinc finger, MIZ-type containing 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    PIAS1 Antibody (YA2217) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2217), targeting PIAS1, with a predicted molecular weight of 72 kDa (observed band size: 76 kDa). PIAS1 Antibody (YA2217) can be used for WB, IHC-P, ICC/IF, FC experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIAS1 using anti-PIAS1 antibody (A01707). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS1 antigen affinity purified polyclonal antibody (Catalog # A01707) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS1 at approximately 72KD. The expected band size for PIAS1 is at 72KD.
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