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PIASx (D-12) Alexa Fluor(r) 790

SC-166494AF790
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPIAS2
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    PIASx (D-12) Alexa Fluor(r) 790
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 790
  • Gene ID9063
  • Target name
    PIAS2
  • Target description
    protein inhibitor of activated STAT 2
  • Target synonyms
    ARIP3, DIP, MIZ1, PIASX, SIZ2, ZMIZ4, E3 SUMO-protein ligase PIAS2, DAB2-interacting protein, E3 SUMO-protein transferase PIAS2, androgen receptor-interacting protein 3, msx-interacting zinc finger protein, protein inhibitor of activated STAT X, zinc finger, MIZ-type containing 4
  • Host
    Mouse
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PIAS2 using anti-PIAS2 antibody (A04130-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human HL-60 whole cell lysates, Lane 6: human Raji whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse testis tissue lysates, Lane 12: mouse Neuro-2a whole cell lysates, Lane 13: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS2 antigen affinity purified polyclonal antibody (Catalog # A04130-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS2 at approximately 68KD. The expected band size for PIAS2 is at 68KD.
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