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Western blot analysis in A549 cell line lysates (35ug/lane) using PIK3AP1 antibody. (C-term). This demonstrates the PIK3AP1 antibody detected the PIK3AP1 protein (arrow).
Western blot analysis in A549 cell line lysates (35ug/lane) using PIK3AP1 antibody. (C-term). This demonstrates the PIK3AP1 antibody detected the PIK3AP1 protein (arrow).
Western blot analysis in A549 cell line lysates (35ug/lane) using PIK3AP1 antibody. (C-term). This demonstrates the PIK3AP1 antibody detected the PIK3AP1 protein (arrow).

PIK3AP1 (C-term) Rabbit Polyclonal Antibody

AP53304PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetPIK3AP1
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PIK3AP1 (C-term) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID118788
  • Target name
    PIK3AP1
  • Target description
    phosphoinositide-3-kinase adaptor protein 1
  • Target synonyms
    BCAP, phosphoinositide 3-kinase adapter protein 1, B cell adaptor protein, B-cell adapter for phosphoinositide 3-kinase, B-cell phosphoinositide 3-kinase adapter protein 1
  • Host
    Rabbit
  • Protein IDQ6ZUJ8
  • Protein Name
    Phosphoinositide 3-kinase adapter protein 1
  • Scientific Description
    PIK3AP1 (C-term) rabbit polyclonal antibody, Aff - Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PIK3AP1 using anti-PIK3AP1 antibody (A08114). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3AP1 antigen affinity purified polyclonal antibody (Catalog # A08114) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3AP1 at approximately 90 kDa. The expected band size for PIK3AP1 is at 90,95,100 kDa.
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Immunofluorescence staining of A549 cells with CSB-PA747806LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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