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Pitx2 Polyclonal Antibody

CAC07937
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPITX2
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Overview

  • Supplier
    Biomatik
  • Product Name
    PITX2 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5308
  • Target name
    PITX2
  • Target description
    paired like homeodomain 2
  • Target synonyms
    ARP1, ASGD4, Brx1, IDG2, IGDS, IGDS2, IHG2, IRID2, Otlx2, PTX2, RGS, RIEG, RIEG1, RS, pituitary homeobox 2, ALL1-responsive protein ARP1, all1-responsive gene 1, homeobox protein PITX2, paired-like homeodomain transcription factor 2, rieg bicoid-related homeobox transcription factor 1, solurshin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99697
  • Protein Name
    Pituitary homeobox 2
  • Scientific Description
    The Pitx2 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PITX2 using anti-PITX2 antibody (A01636-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: rat heart tissue lysates, Lane 8: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITX2 antigen affinity purified polyclonal antibody (Catalog # A01636-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PITX2 at approximately 35KD. The expected band size for PITX2 is at 35KD.
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