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ProductsBack/PITX2 / RGS Antibody

PITX2 / RGS Antibody

LS-C408366
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPITX2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PITX2 / RGS Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 30kDa/35kDa, while the observed MW by Western blot was 34kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 30kDa/35kDa, while the observed MW by Western blot was 34kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5308
  • Target name
    PITX2
  • Target description
    paired like homeodomain 2
  • Target synonyms
    all1-responsive gene 1; ALL1-responsive protein ARP1; ARP1; ASGD4; Brx1; homeobox protein PITX2; IDG2; IGDS; IGDS2; IHG2; IRID2; Otlx2; paired-like homeodomain transcription factor 2; pituitary homeobox 2; PTX2; RGS; RIEG; rieg bicoid-related homeobox transcription factor 1; RIEG1; RS; solurshin
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PITX2 using anti-PITX2 antibody (A01636-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: rat heart tissue lysates, Lane 8: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PITX2 antigen affinity purified polyclonal antibody (Catalog # A01636-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PITX2 at approximately 35KD. The expected band size for PITX2 is at 35KD.
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