IHC-P analysis of colon adenocarcinoma tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
![IHC-P analysis of human ovary adenocarcinoma tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83823/GTX83823_1863_IHC-P_w_23061420_577.webp "IHC-P analysis of human ovary adenocarcinoma tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of liver carcinoma tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83823/GTX83823_1864_IHC-P_w_23061420_125.webp "IHC-P analysis of liver carcinoma tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of kidney tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83823/GTX83823_1865_IHC-P_w_23061420_684.webp "IHC-P analysis of kidney tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of pancreas tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83823/GTX83823_1866_IHC-P_w_23061420_587.webp "IHC-P analysis of pancreas tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![IHC-P analysis of prostate tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX83823/GTX83823_1867_IHC-P_w_23061420_499.webp "IHC-P analysis of prostate tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50")
![FACS analysis of Jurkat cells using GTX83823 PKA R2 antibody [1A7]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83823/GTX83823_192_FACS_w_23061420_480.webp "FACS analysis of Jurkat cells using GTX83823 PKA R2 antibody [1A7]. Red : Primary antibody Blue : Negative control antibody")
![WB analysis of wild type and PKA R2 knock out HeLa cell lysate(10microg per lane) using GTX83823 PKA R2 antibody [1A7]. Dilution : 1:2000](https://www.genetex.com/upload/website/prouct_img/normal/GTX83823/GTX83823_3927_20181228_WB_w_23061420_672.webp "WB analysis of wild type and PKA R2 knock out HeLa cell lysate(10microg per lane) using GTX83823 PKA R2 antibody [1A7]. Dilution : 1:2000")
![WB analysis of HEK293T cells transfected with PKA R2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83823 PKA R2 antibody [1A7]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83823/GTX83823_3928_WB_w_23061420_183.webp "WB analysis of HEK293T cells transfected with PKA R2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83823 PKA R2 antibody [1A7]. Loading : 5 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with PKA R2 plasmid using GTX83823 PKA R2 antibody [1A7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83823/GTX83823_835_ICCIF_w_23061420_369.webp "ICC/IF analysis of COS7 cells transiently transfected with PKA R2 plasmid using GTX83823 PKA R2 antibody [1A7].")
IHC-P analysis of colon adenocarcinoma tissue using GTX83823 PKA R2 antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
PKA R2 antibody [1A7]
GTX83823
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRKAR2A
Overview
- SupplierGeneTex
- Product NamePKA R2 antibody [1A7] - KO/KD, Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1A7
- Concentration0.5 mg/ml
- ConjugateUnconjugated
- Gene ID5576
- Target namePRKAR2A
- Target descriptionprotein kinase cAMP-dependent type II regulatory subunit alpha
- Target synonymscAMP-dependent protein kinase regulatory subunit RII alpha; cAMP-dependent protein kinase type II-alpha regulatory subunit; PKR2; PRKAR2; protein kinase A, RII-alpha subunit; protein kinase, cAMP-dependent, regulatory subunit type II alpha
- HostMouse
- IsotypeIgG1
- Protein IDP13861
- Protein NamecAMP-dependent protein kinase type II-alpha regulatory subunit
- Scientific DescriptionType II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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